Difference between revisions of "Projects"
From GenPlay, Einstein Genome Analyzer
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=== Identification of a BET Family Bromodomain/Casein Kinase II/TAF-Containing Complex as a Regulator of Mitotic Condensin Function === | === Identification of a BET Family Bromodomain/Casein Kinase II/TAF-Containing Complex as a Regulator of Mitotic Condensin Function === | ||
| − | + | ====Abstract: ==== | |
| + | Condensin is a central regulator of mitotic genome structure with mutants showing poorly condensed chromosomes and profound segregation defects. Here, we identify NCT, a complex comprising the Nrc1 BET-family tandem bromodomain protein (SPAC631.02), casein kinase II (CKII), and several TAFs, as a regulator of condensin function. We show that NCT and condensin bind similar genomic regions but only briefly colocalize during the periods of chromosome condensation and decondensation. This pattern of NCT binding at the core centromere, the region of maximal condensin enrichment, tracks the abundance of acetylated histone H4, as regulated by the Hat1-Mis16 acetyltransferase complex and recognized by the first Nrc1 bromodomain. Strikingly, mutants in NCT or Hat1-Mis16 restore the formation of segregation-competent chromosomes in cells containing defective condensin. These results are consistent with a model where NCT targets CKII to chromatin in a cell-cycle-directed manner in order to modulate the activity of condensin during chromosome condensation and decondensation. | ||
| − | + | ====Article: ==== | |
| + | [http://www.cell.com/cell-reports/fulltext/S2211-1247(14)00063-1 http://www.cell.com/cell-reports/fulltext/S2211-1247(14)00063-1] | ||
'''GenPlay Project: '''[http://genplay.einstein.yu.edu/library/projects/CellReports-2014/Kim_CellRep_2014.gppf] | '''GenPlay Project: '''[http://genplay.einstein.yu.edu/library/projects/CellReports-2014/Kim_CellRep_2014.gppf] | ||
== Projects from tutorials == | == Projects from tutorials == | ||
Revision as of 17:43, 18 March 2014
This page contains GenPlay projects available for downloads. This projects illustrate the type of data analysis and visualization that can be done with GenPlay. There are two sections: the first one contains projects that were used as supplementary data of articles published in scientific journals. The second section contains the projects created for the Tutorial section of this website.
Projects from publish work
Allele-Specific Genome-wide Profiling in Human Primary Erythroblasts Reveal Replication Program Organization
Identification of a BET Family Bromodomain/Casein Kinase II/TAF-Containing Complex as a Regulator of Mitotic Condensin Function
Abstract:
Condensin is a central regulator of mitotic genome structure with mutants showing poorly condensed chromosomes and profound segregation defects. Here, we identify NCT, a complex comprising the Nrc1 BET-family tandem bromodomain protein (SPAC631.02), casein kinase II (CKII), and several TAFs, as a regulator of condensin function. We show that NCT and condensin bind similar genomic regions but only briefly colocalize during the periods of chromosome condensation and decondensation. This pattern of NCT binding at the core centromere, the region of maximal condensin enrichment, tracks the abundance of acetylated histone H4, as regulated by the Hat1-Mis16 acetyltransferase complex and recognized by the first Nrc1 bromodomain. Strikingly, mutants in NCT or Hat1-Mis16 restore the formation of segregation-competent chromosomes in cells containing defective condensin. These results are consistent with a model where NCT targets CKII to chromatin in a cell-cycle-directed manner in order to modulate the activity of condensin during chromosome condensation and decondensation.
Article:
http://www.cell.com/cell-reports/fulltext/S2211-1247(14)00063-1
GenPlay Project: [1]